Supramolecular Materials for 3D Cell Culture of Induced Pluripotent Stem Cells and their Derivative
Cells that make up tissues are situated within a complex three-dimensional (3D) structure known as the extracellular matrix (ECM). There is growing appreciation for the role the ECM plays in regulating cell behavior and 3D cell cultures (particularly hydrogels) have been developed to allow for closer representation of a cell's natural environment and growth pattern. It is known as the mechanical, structural, and compositional cues, either alone or in concert, drastically altered cell function (Caliari & Burdick, 2016).
The invention describes a self-assembled squaramide-based tripodal hydrogel based on hydrogen bonding and pi-stacking for use as a material in cell culture, particularly for the culture or pluripotent stem cells.
This technology involves a hydrogel material that can easily be outfitted with various biochemical and biophysical cues that mimic the natural extracellular matrix in vivo for the in vitro culture of cells in 3D.
The inherently modular nature of the materials permits mixing and matching or biochemical and biophysical cues in a user-defined manner. Because the capacity to easily and gently seed and release cells from the matrix, sensitive cell types such as induced pluripotent stem cells and their derivatives can be cultivated within the matrix.
Details and State of Development:
The scientists are currently working on methods to modulate the mechanical properties of these materials to broaden their range in the field of 3D cell culture and exploring a range of peptides for maintenance and differentiation or iPSCs to numerous cell types.