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Device to Remove Unwanted Solvents from Biochemical Samples

Background

Biochemical analysis of complex mixtures is of great importance in various fields of application. A combination of multiple analytical techniques, such as LC-MS, GC-MS and LC-NMR, is often needed to achieve sufficient molecular separation and enrichment. The combination of LC with NMR is not straightforward, certainly not for polar molecules. Scientists at Leiden University have developed an efficient and robust interface between LC and NMR to overcome this problem.

Technology Overview

The exchange of solvent is achieved by the controlled evaporation of a (hanging) droplet using a machine vision feedback loop (Figure 1). The apparatus was successfully tested for a multitude of samples containing volatile, thermosensitive, polar and nonpolar analytes.

 

Details and State of Development:

- Fully operational system with proof of principle for use in two-dimensional LCxLC and LC-NMR.

 

Applications


Multidimensional LC

Solvent switching: e.g. NMR

Sample preconcentration

Active component discovery in complex mixtures run in isocratic and gradient systems

The technique can be coupled to spotting techniques such as MALDI-MS and Thin Layer Chromatography










 

Opportunity

Leiden University is looking for partners for further (joint) development of the device and is looking to license this powerful technology to commercial part(ies).

Keywords: analytical (bio)chemistry, liquid chromatography, NMR

Key benefits

  • Multidimensional LC
  • Solvent switching: e.g. NMR
  • Sample preconcentration
  • Active component discovery in complex mixtures run in isocratic and gradient systems
  • The technique can be coupled to spotting techniques such as MALDI-MS and Thin Layer Chromatography

Luris reference number

INV-034.063

Patent status

A patent application has been filed.

Data available on request

A scientific publication is available on request.

Further information

Frits Fallaux Business Developer +31-71-527 2517 f.j.fallaux@luris.nl